Isolation and identification of cytotoxic compounds from a fruticose lichen Roccella montagnei, and it's in silico docking study against CDK-10

ABSTRACT Roccella montagnei Bél. belongs to lichen family Roccelleceae growing luxuriantly along the coastal regions of India. As Roccella has been shown to be bioactive, we prepared methanolic extract and assessed its anticancer potential. The methanolic extract showed significant in vitro cytotoxic activity against four human cancer cell lines such as colon (DLD-1, SW-620), breast (MCF-7), head and neck (FaDu). This prompted us to isolate bioactive compounds through column chromatography. Two compounds roccellic acid and everninic acid have been isolated, out of which everninic acid is reported for the first time. Both the compounds have been tested for in vitro cytotoxic activity in which roccellic acid showed strong anticancer activity as compared to the everninic acid. Cyclin Dependent Kinase (CDK-10) contributes to proliferation of cancer cells, and aberrant activity of these kinases has been reported in a wide variety of human cancers. These kinases therefore constitute biomarkers of proliferation and attractive pharmacological targets for development of anticancer therapeutics. Therefore both the isolated compounds were tested for in silico molecular docking study against Cyclin Dependent Kinase isomer enzyme to support the cytotoxic activity.

Effect of a non steroidal tamoxifen on the gonad and sex differentiation in Nile tilapia, Oreochromis niloticus.

The effect of different doses of anti-estrogenic chemical was studied in a cichlid Oreochromis niloticus on mortality, growth, sexual development and sex differentiation. Percent mortality (5, 10 and 10) appeared to be dose-dependent. All groups of tamoxifen (TA) treated fishes exhibited significant growth as compared to the controlled fishes. Immature fishes treated with TA at 200 mg l-1, showed lower gonado-somatic-index (GSI) (male 0.137; female 0.118) than control group (male 0.138; female 0.122) of fishes. Ovarian and testicular differentiation was adjudged by the appearance of the ovarian cavity (ovary) and the efferent (sperm) duct (testis), respectively. Sex and sexual maturity of individual fishes were determined using standard histological method. Tamoxifen treatment to the fry (yolk sac absorbed stage) of Oreochromis niloticus in the dose of 200 mg l-1 for 60 days produced 90% males and 1% intersex population. Results suggested masculinizing action of tamoxifen reversing the sex of genetic female tilapia.

Effect of examination stress on mood, performance and cortisol levels in medical students.

Stress produces definable mental and physiological reactions in the body. Mild stress is beneficial in cognitive tasks and performance but persistently high stress may lead to neuropsychiatric illnesses like anxiety and depression. Examinations act as stressor and activate hypothalamicpituitary adrenal axis causing an increase in cortisol level, which is reflected in saliva. Present study was done on 35 medical students. Their mood parameters were assessed, using Depression Anxiety Stress Scale (DASS) scoring, and salivary cortisol levels using quantitative ELISA. Subjects were evaluated for mood parameters two times, one during relaxed state (with no examinations in preceding 2 weeks and in coming 2 weeks) and another during stressed state (on the day of viva voce examination). The levels of mood parameters and salivary cortisol were significantly raised during examination stress. The changes in stress level significantly correlated with change in levels of anxiety and salivary cortisol though there was no significant effect on the performance. Males and females showed similar changes in mood parameters. This study suggests that as examinations act as unavoidable stressors, the medical educators as well as students should be made aware of the negative consequences of stress faced during medical training. Efficient relaxation program as well as counseling services should be provided to stressed students so that they are able to cope better with examination stress.

Gap between awareness and practices regarding maternal and child health among women in an urban slum community.

OBJECTIVE: To investigate the knowledge of ever-married women regarding maternal and child health and to assess existing gap between awareness and practice levels of lactating mothers. METHODS: Community based cross sectional study with 283 ever-married women in the reproductive age. RESULTS: Low levels of awareness and practice of TT immunization (74.2% awareness, 58.1% practice), five-cleans (31.9% awareness and 14.4% practice), trained birth attendant (69.6% awareness 39.1% practice), and post-natal care (75.4% awareness and 51.0% practice) among lactating mothers. Knowledge regarding optimal infant and young child feeding practices was very poor: initiation of breast-feeding within six hours (17.4%), colostrum feeding (34.8%), exclusive breastfeeding (5.8%) and significant gaps between knowledge and practice were observed. Highly significant difference (P<0.001) between proportions of women aware of ICDS (59.4%) and of beneficiaries (21.7%). High levels of awareness regarding reproductive health parameters except for contraception: desire for two children (81.6%), legal ages at marriages for girls (84.5%), desired birth interval of three or more years (71.7%). CONCLUSION: Wide gaps exist between awareness and practices related with MCH due to non-adoption of knowledge into actual practice, except some selected components lacking in both knowledge as well as practice. Need for bridging the existing gaps avoiding socio-cultural barriers and misconceptions prevailing in the community and by promoting and protecting healthy MCH care practices.

Challenges in the diagnosis of post kala-azar dermal leishmaniasis.

Post kala-azar dermal leishmaniasis (PKDL) is a dermatosis that occurs as a sequel of visceral leishmaniasis (VL). Elimination of VL requires detection and treatment of PKDL, necessarily because of its capacity to serve as a reservoir for the causative parasite, Leishmania donovani. Diagnosis of PKDL presents a challenge due to low parasite burden in the lesions. In this article we have reviewed the recent advances in the development of immunological and molecular methods for diagnosis of PKDL.